Happy Friday dvm360 readers! Did you know that the dvm360 editorial team has hit the road and is attending the 2025 American College of Veterinary Internal Medicine (ACVIM) Forum in Louisville Kentucky. You can check out our coverage of the event on dvm360’s dedicated 

, along with coverage from other conferences include Fetch, AVMA Convention, and VMX!

Besides covering the ACVIM Forum, here is what dvm360’s team covered this week:

Earlier this week, Lincoln Memorial University, one of the biggest veterinary schools in the United States, 

 that they have filed a lawsuit against the American Veterinary Medical Association, stating that they are violating antitrust laws in the United States.

 is now available online! This article takes a deep dive into the emotional wellbeing of patients and how their behavior can be signs of distress, plus what ways it can be managed with interventions.

 of The Vet Blast Podcast presented by dvm360, our host Adam Christman, DVM, MBA, welcomed Jennifer Quammen, DVM, MPH, to share more on her careers in veterinary medicine, plus why the industry needs more diverse perspectives, inclusivity, and sustainability.

 of Fetch Nashville? You can check out this exclusive content available on dvm360.com here! From lecture highlights to interviews with key industry leaders and more, you can relive your time and the conference, or see what you missed, all 

 with dvm360 at the 2025 ACVIM Forum in Louisville, Kentucky, Frederick R. Appelbaum, MD, shared the pivotal role that canine patients play in advancing human and veterinary medicine, which he highlighted in his lecture on bone marrow transplantation.

Articles

Wrap up: LMU sues AVMA, and other veterinary news 

Dogs with diabetes exhibited alterations distinct from those seen in human type 1 diabetes, which is linked to hypovitaminosis D, in a study evaluating vitamin D metabolism in these canines. Results of this study, which was the first to use a standardized diet to investigate vitamin D metabolism in dogs with diabetes, were presented with a poster at the 2025 American College of Veterinary Internal Medicine (ACVIM) Forum in Louisville, Kentucky.

Hypovitaminosis D is linked to human insulin resistance and an increased risk of type 1 and type 2 diabetes. However, an association between hypovitaminosis D and diabetes in dogs is unclear, although there is a well-established role of hypovitaminosis D in canine autoimmune and inflammatory diseases, according to investigators.

The study’s objective was to assess whether dogs with diabetes exhibit alterations in serum vitamin D status and to help set a standard diet for these affected canines, according to investigators. “The great finding was that, different from diabetes in humans, the serum vitamin D was not decreased; it was increased,” Camila S. Torelli, MS, the presenting author and a professor at UNIFACCAMP in Sao Paulo, Brazil, said in a 

In fact, investigators found that dogs with diabetes had significantly higher mean serum 24,25-(OH)

 = .007) in the study. Meanwhile, no significant differences were found in age or median serum calcidiol concentrations between groups (

The study enrolled 27 dogs with diabetes and free from other diseases, as well as 19 healthy canines. According to investigators, the dogs with diabetes had a 60-day stabilization period. During this time, they received NPH insulin and a diet formulated for canine diabetes.

Following the stabilization period, the dogs with diabetes were fed the same diet for 2 months, whereas healthy dogs in the study were fed the same diet for 60 days. Investigators analyzed serum calcidiol and 24,25-(OH)

 and used Wilcoxon or t-tests to compare dogs with diabetes with healthy canines.

Investigators noted the findings align with human juvenile insulin-dependent diabetes, for which increased calcidiol and serum 24,25-(OH)

 concentrations have also been observed. In the poster presentation, they wrote that these elevated serum concentrations found may be caused by mitochondrial stimulation of 24-hydroxylase by diabetes metabolites, a scenario that has been reported in insulin-dependent rats.

Torelli said these study results can lead to more studies on diabetes and metabolism, as well as other perspectives for treatment and diagnosis. “Diabetes is definitely something that people are interested in learning more about. It's revealing to be something of big complexity, with diagnosis, treatment, [and] prognosis,” she concluded.

Pereira EF, Torelli C, Torres de Almeida Villar S, et al. Serum vitamin D metabolites in diabetic dogs. Presented at: 2025 American College of Veterinary Internal Medicine (ACVIM) Forum; June 18-21, 2025; Louisville, KY.

Results were presented at the 2025 American College of Veterinary Internal Medicine (ACVIM) Forum in Louisville, Kentucky.

Researchers study vitamin D metabolism in dogs with diabetes

A researcher at the Virginia-Maryland College of Veterinary Medicine talks about her work validating a food frequency questionnaire for use in canine nutrition research in this interview with dvm360

Validating a dietary survey tool for dogs 

What is a food frequency questionnaire (FFQ) and how can one be used in veterinary medicine? At the 2025 American College of Veterinary Internal Medicine (ACVIM) Forum in Louisville, Kentucky, 

Janice O’Brien, DVM, MPH, DACVMP, and a United States Army veteran

, presented a study in which she and a team of researchers sought to validate the semi-quantitative food frequency questionnaire used by the Dog Aging Project—a nationwide study on aging and health in dogs.  

O’Brien, currently a PhD student at the Virginia-Maryland College of Veterinary Medicine in Blacksburg, Virginia, spoke with 

 during the 2025 ACVIM Forum about her research, FFQs, and their application to canine research.

Below is a partial transcript of the interview:

So, food frequency questionnaires are something that [are used] in the human research side an awful lot to study how large populations of people might eat. And so, it's kind of exactly what it sounds like.

So, it's what food items [are consumed] and how frequently [they are] eaten. So, for example, it might have a list of different meats—so sausages, pork, bacon within that. And how frequently it's eaten, if it's eaten several times a day, if it's eaten once a day, if it's eaten once a week, etc., and so it's just that frequency for each item.

And then they use that to study, at large population levels, this is where you get those studies that say people that eat more fruits and vegetables or who consume more fiber generally, have, you know, better cardiac outcomes or something like that. That's where those types of studies come from, is from those food frequency questionnaires.

And we wanted to do that on the dog side because, well A) that format is what the Dog Aging Project diet survey is formatted like. So, we wanted to validate, 

is this accurately recording the information we would like it to be recording?

This transcript was lightly edited for improved clarity

Videos

A researcher at the Virginia-Maryland College of Veterinary Medicine talks about her work validating a food frequency questionnaire for use in canine nutrition research

This podcast episode is sponsored by Antech Diagnostics

On this special episode of the Vet Blast Podcast presented by dvm360, Michelle Evason, BSc, DVM, DACVIM (SAIM), MRCVS, joins our host Adam Christman, DVM, MBA, to preview Antech’s research highlights at the 2025 ACVIM Forum, including all 11 submitted abstracts being accepted. She discusses new PCR panels for vector-borne diseases, head-to-head diagnostic comparisons, practical tools for general practice, and more.

Below is a partial transcript, edited lightly for clarity 

So many different conversations to have on what we choose and the why behind the recommendation, and is there one specific piece of research you're most interested in from ACVIM?

Michelle Evason, BSc, DVM, DACVIM (SAIM), MRCVS: 

You know, it's really hard to choose. I think for me, one of the things that I'm really excited about is a presentation that was submitted from the team,

[Christian M. Leutenegger, DrMedVet, BSc, PhD, FVH] will be presenting on the topic of PCR testing for some of these infectious diseases, vector-borne specifically. We are launching, and we actually, we have launched at this time, an updated and expanded PCR panel that's specifically available to look at genetic material from 21 vector-borne disease pathogens, and also includes a marker for atovaquone resistance, which we know is a concern now in cats, for the treatment of for treatment planning, for cytauxzoon felis, and for dogs, for babesia gibsoni and so Christian, Dr Lutnegger, will be presenting his work on that topic, since this test was designed and executed by his team, and we're proud to launch it. 

Speaking to the practicality by comparing the panel to the one that most of us specialists regard as, I hate to use the term gold standard, but the panel to use when you're looking for evidence of genetic material on these vector-borne diseases, which is NC State's. And so he'll be presenting a head-to-head comparison at the Forum this year, and I'm really excited to see that one, because, again, I think that's a truly innovative test. 

 It really is. You know, when you're talking, I was like, if you look at 5 years ago, when I was doing PCR testing in practice, we didn't have hardly any of that at all. And look how far we've come. That's really exciting, right there

Podcasts

Michelle Evason, BSc, DVM, DACVIM (SAIM), MRCVS, previews Antech’s research at the 2025 ACVIM Forum, highlighting new diagnostic tools and more

ACVIM and hot research: Vector-borne disease and more 

Ingredients are diverse in home-prepared food for dogs, according to the results of a recent study. Furthermore, only 5% of the 1765 cases studied potentially met maintenance diet standards set by the Association of American Feed Control Officials (AAFCO).

These and other insights were presented by the study’s lead author, Janice O’Brien, DVM, a PhD student in dog nutrition epidemiology at Virginia-Maryland College of Veterinary Medicine in Blacksburg, Virginia, at the 2025 American College of Veterinary Internal Medicine (ACVIM) Forum in Louisville, Kentucky. She shared that about 25% of diets for pets enrolled in the study were partially balanced (1 to 10 nutrient imbalances), and 52% were unbalanced (10 or more nutrient imbalances), while approximately 17% could not be categorized.

O’Brien, who previously served in the US Army Veterinary Corps and has practiced veterinary medicine in a small animal clinic, said that adding vitamins and minerals to home-prepared foods is necessary in the majority of cases. “There's probably some sort of supplement that is going to have to be added, and it needs to be added all the time,” O’Brien said in a 

Overall, the study sought to discover whether a complete diet can be achieved with home-prepared meals, based on ingredients listed by pet owners. The results showed the most common animal sources reported by pet owners were chicken, beef and turkey. The most common ingredients with percentage of diets they were contained in as follows

In another recent study—published in ACVIM’s 

Medicine—for which O’Brien was also an author, investigators found an association between home-cooked diets and higher odds of gastrointestinal, renal, and hepatic diseases when compared to an extruded diet.

 However, there is a lack of previous research about how pet owners are feeding their dogs at home.

According to data from the Dog Aging Project, which has supported both studies, about 6% of the 40,307 dogs in a subset sample had home-prepared foods, either cooked or raw, as their primary diet with another 25% of dogs in the analysis having home-prepared foods as a secondary diet component.

 O’Brien noted that many pet owners might be feeding a commercial kibble diet, but then adding a home-cooked food on top and mixing it in.

O’Brien emphasized that pet owners should be closely following the recipes they use for their animals’ meals. “If you have a recipe that's formulated, make sure that you continue doing that exact recipe all the time, because otherwise, if you drift away from what it initially was, that's going to result in changes to the balance of the diet,” she said.

Veterinary professionals should keep in mind that pet owners really care about their dogs, O’Brien said. “They're really intending to do the best thing for them. I don't think they're intending to do something that's bad, and I think it's just a way that we as a profession can maybe communicate that better,” she concluded.

O’Brien J, Ruple A, Tolbert K. Abstract NM04: Home-prepared diets for companion dogs are made from diverse ingredients and few are balanced. Presented at: American College of Veterinary Internal Medicine (ACVIM) Forum; June 18-21, 2025: Louisville, KY.

Ortiz AV, Luo I, O’Brien J, et al. Association between diet type and owner-reported health conditions in dogs in the Dog Aging Project. 

About the diet survey. Dog Aging Project. 2024. Accessed June 19, 2025. 

https://data.dogagingproject.org/HlesDiet?year=2024#primary-diet

Results of a recent research found a wide variety of ingredients are being used to feed pets.

Study: Few home-prepared diets are balanced

Researchers and veterinarians have been seeing an emerging detection and range expansion of E

around the globe. The rise in detection of this parasitic tapeworm not only poses a concern for individual patients, but it also carries One Health implications, presenting an environmental and public health risk. At the 2025 American College of Veterinary Internal Medicine Forum in Louisville, Kentucky, a group of specialists (Michelle Evason, DVM, BSc, DACVIM, MRCVS, global director of veterinary clinical education at Antech Diagnostics/Mars Petcare Science and Diagnostics in Prince Edward Island, Canada; Christian M. Leutenegger, DrMedVet, BSc, PhD, FVH, vice president of research and development at Antech Diagnostics/Mars Petcare Science and Diagnostics in Fountain Valley, California; Roger Ramirez-Barrios, DVM, MSc, PhD, DACVM (Parasitology), clinical professor at Virginia-Maryland College of Veterinary Medicine in Blacksburg, Virginia; Michael R. Lappin, DVM, PhD, DACVIM (SAIM), professor at Colorado State University in Fort Collins, Colorado) led a lecture on “

and Other Emerging One Health Concerns in Dogs,” which covered the geographical expansion of the parasite in the US and Canada and recent detections in these regions.

Domestic dogs and wild canids such as coyotes, foxes, and wolves serve as definitive hosts of 

 Meanwhile, rodents act as the typical intermediate hosts of the parasite—during which it will be in its larval (or metacestode) stage—whereas humans and dogs can serve as “dead-end” hosts following accidental ingestion of 

According to the presenters, dogs can also autoinfect, most commonly through grooming.

If a dog or wild canid consumes a rodent with 

, the adult form of the parasite will develop in its small intestine. The animal will then shed the parasite eggs in their feces.

“A friend of mine and I usually refer to these eggs as little time bombs, right? Ticking time bombs, in part, because not only are they immediately infective, but they are environmentally resistant,” Evason said during the presentation, explaining that eggs can survive harsh weather conditions, such as Alaskan or Canadian winters.

ACVIM and hot research: Vector-borne disease and more

can cause alveolar echinococcosis (AE), one of the most lethal helminthic infections in humans, dogs, and rodents if left untreated.

According to Ramirez-Barrios, it is not exactly known how long the incubation period of AE is, but it is believed to be as long as 20 or 25 years.

 He noted that survival is poor without treatment, as by the time many cases are detected, it is too late for effective intervention.

In the US, Northern Virginia has reported 3 positive cases of 

 Pennsylvania, meanwhile, has seen 13 positive cases in coyotes, with detections in coyotes in Georgia as well.

 In Canada, the parasite has also been detected in British Columbia and Alberta.

“We don't know why, but we have only found foxes [positive for 

] in Virginia and only [positive] coyotes in Pennsylvania. But we already know that [

]—just to give a brief background—has several haplotypes. Historically, in the [US], the haplotypes found [have been] the North American haplotype, and they have been present only in the central states in the United States,” Ramirez-Barrios said.

“However, when [a] former parasitologist...found [a] dog with [AE], it was a new European haplotype. And then...all the foxes and coyotes [with AE] that we have found in eastern US [have carried] the European haplotype, which is really more pathogenic...than the North American haplotype,” Ramirez-Barrios said.

According to Ramirez-Barrios, the first human case of AE in the Eastern US was reported in 2021 from Vermont, around the same time 

was detected in foxes in Northern Virginia.

After contacting the authors of the Vermont case, Ramirez-Barrios and his team began a molecular analysis of the parasite and discovered a second human case. Phylogenetic testing from his team revealed that the parasite from both human cases and the infected foxes shared the exact same European haplotype.

According to Ramirez-Barrios, one of the human patients grew up in Maryland hunting foxes, near the counties where foxes positive for 

 were found. “When we did the phylogenetic [testing] of the 3 different isolates that we found, we discovered that they were exactly the same haplotype, so they were genetically the same organism,” Ramirez-Barrios said. “That's when we published [a] report of the 2 human cases and their link with wildlife, which demonstrates that the parasite is actively transmitting in Eastern US, especially the upper haplotype, which is emerging.”

In the session, Evason emphasized the need for an interdisciplinary One Health approach for managing gastrointestinal parasites like 

. She highlighted the need for better communication with pet owners.

“The goal is to educate our clients that they need to pick up [their dog’s] feces, especially in their backyards,” Ramirez-Barrios said, noting that not picking it up poses a contamination risk to the surrounding environment.

“I am educating new generations of veterinarians to educate their clients,” he said. “Right now, [picking up dog feces] is one of the best things that we have to control parasites.”

“In [future] years, it's going to be the only thing that we will have. So, if we don't start now, it's going to [cause] a big problem in the future,” he concluded.

Evason M, Leutenegger C, Ramirez-Barrios R, Lappin M. Echinococcus multilocularis and Other Emerging One Health Concerns in Dogs and Cats. Presented at: American College of Veterinary Internal Medicine Forum; Louisville, KY; June 18, 2025.

A session at the 2025 ACVIM Forum covered cases of echinococcus multilocularis and its expanding presence in North America, highlighting the One Health risks for both veterinary and human populations.